Potent suppression of HIV-1 replication in humans by T-20, a peptide inhibitor of gp41-mediated virus entry.

Academic Article

Abstract

  • T-20, a synthetic peptide corresponding to a region of the transmembrane subunit of the HIV-1 envelope protein, blocks cell fusion and viral entry at concentrations of less than 2 ng/ml in vitro. We administered intravenous T-20 (monotherapy) for 14 days to sixteen HIV-infected adults in four dose groups (3, 10, 30 and 100 mg twice daily). There were significant, dose-related declines in plasma HIV RNA in all subjects who received higher dose levels. All four subjects receiving 100 mg twice daily had a decline in plasma HIV RNA to less than 500 copies/ml, by bDNA assay. A sensitive RT-PCR assay (detection threshold 40 copies/ml) demonstrated that, although undetectable levels were not achieved in the 14-day dosing period, there was a 1.96 log10 median decline in plasma HIV RNA in these subjects. This study provides proof-of-concept that viral entry can be successfully blocked in vivo. Short-term administration of T-20 seems safe and provides potent inhibition of HIV replication comparable to anti-retroviral regimens approved at present.
  • Published In

  • Nature Medicine  Journal
  • Keywords

  • Adult, Anti-HIV Agents, CD4 Lymphocyte Count, Dose-Response Relationship, Drug, Enfuvirtide, HIV Envelope Protein gp41, HIV Infections, HIV-1, Half-Life, Humans, Metabolic Clearance Rate, Peptide Fragments, Virus Replication
  • Digital Object Identifier (doi)

    Author List

  • Kilby JM; Hopkins S; Venetta TM; DiMassimo B; Cloud GA; Lee JY; Alldredge L; Hunter E; Lambert D; Bolognesi D
  • Start Page

  • 1302
  • End Page

  • 1307
  • Volume

  • 4
  • Issue

  • 11