Transmission of Chlamydia trachomatis among Sex Partners Assessed by Polymerase Chain Reaction

Academic Article

Abstract

  • The prevalence of Chlamydia infection in 95 sex partners was determined by both polymerase chain reaction (PCR) and cell culture. Thirty-three (18%) of 186 specimens were positive by culture and 61 (33%) were positive by PCR-EIA. PCR was positive in 75% (21/28) of male partners of PCR-positive women compared with culture, which was positive in only 45% (9/19) of male partners of culture-positive women (P =.053). For female partners of infected men, the difference was less marked. PCR was positive in 58% (21/36) of female partners of infected men versus culture, which was positive in 56% (15/36) offemale partners of culture-positive men. The correlation of PCR between partners and sequence analysis of Chlamydia DNA showing the same sequence from sex partners of 7 couples support the accuracy of the assay. These data suggest that PCR is more sensitive than culture for detection of Chlamydia trachomatis, particularly for male partners of infected women. © 1993 by The University of Chicago.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Viscidi RP; Hobo L; Hook EW; Quinn TC
  • Start Page

  • 488
  • End Page

  • 492
  • Volume

  • 168
  • Issue

  • 2