Severe phenotype in mice with termination mutation in exon 2 of cystic fibrosis gene

Academic Article

Abstract

  • Mice with a termination codon mutation in exon 2 of the cystic fibrosis (CF) gene were generated using homologous recombination in embryonic stem cells. Animals homozygous for the mutant allele display a severe intestinal phenotype similar to that previously reported for CF mutant mice. The null nature of this allele was demonstrated by the absence of detectable wild-type mRNA, by the absence of detectable CFTR in the serous gland collecting ducts of salivary tissues, and by the lack of cAMP-mediated short-circuit current responses in colonic epithelium of mutant animals. © 1995 Plenum Publishing Corporation.
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    Digital Object Identifier (doi)

    Author List

  • Hasty P; O'Neal WK; Liu KQ; Morris AP; Bebok Z; Shumyatsky GB; Jilling T; Sorscher EJ; Bradley A; Beaudet AL
  • Start Page

  • 177
  • End Page

  • 187
  • Volume

  • 21
  • Issue

  • 3