Assessment of CFTR localisation in native airway epithelial cells obtained by nasal brushing.

Academic Article

Abstract

  • Reliable methods for determining the localisation of mutant CFTR protein in native cells from CF individuals are necessary to allow the degree of mislocalisation of any genotype to be defined and to assess the effect of therapeutic agents on CFTR trafficking. Here, we present procedures for obtaining ciliated epithelial cells from CF patients by nasal brushing and a description of protocols for immunolocalisation of CFTR. The protocols are a consensus, following comparison of some aspects of methods currently used in the authors' laboratories.
  • Published In

    Keywords

  • Cystic Fibrosis, Cystic Fibrosis Transmembrane Conductance Regulator, Epithelial Cells, Histocytological Preparation Techniques, Humans, Respiratory Mucosa, Specimen Handling
  • Digital Object Identifier (doi)

    Author List

  • Harris CM; Mendes F; Dragomir A; Doull IJM; Carvalho-Oliveira I; Bebok Z; Clancy JP; Eubanks V; Sorscher EJ; Roomans GM
  • Start Page

  • 43
  • End Page

  • 48
  • Volume

  • 3 Suppl 2