Polyacrylamide gel analysis of polypeptides in gene transfer cell lines.

Academic Article

Abstract

  • Differential polypeptide expression in gene transfer cell lines of limited genetic complexity was analyzed as a gene mapping strategy. Subcellular fractionation preceding two-dimensional gel electrophoretic analysis simplified protein patterns and revealed subcellular location of differentially expressed polypeptides. As a model system, human MET oncogene polypeptide was identified in gene transfer lines by this approach. Genes encoding five putative human proteins were identified and provisionally assigned to chromosomal region 7q21-31 or to chromosome 1.
  • Authors

    Published In

    Keywords

  • Amino Acids, Animals, Cell Line, Chromosome Mapping, Chromosomes, Human, Pair 7, Electrophoresis, Gel, Two-Dimensional, Humans, Isotope Labeling, Mice, Molecular Weight, Peptides, Protein Processing, Post-Translational, Subcellular Fractions, Transfection
  • Authorlist

  • Strong TV; Barker PE; Anderson SJ
  • Start Page

  • 341
  • End Page

  • 349
  • Volume

  • 16
  • Issue

  • 4