Polyacrylamide gel analysis of polypeptides in gene transfer cell lines

Academic Article

Abstract

  • Differential polypeptide expression in gene transfer cell lines of limited genetic complexity was analyzed as a gene mapping strategy. Subcellular fractionation preceding two-dimensional gel electrophoretic analysis simplified protein patterns and revealed subcellular location of differentially expressed polypeptides. As a model system, human MET oncogene polypeptide was identified in gene transfer lines by this approach. Genes encoding five putative human proteins were identified and provisionally assigned to chromosomal region 7q21-31 or to chromosome 1. © 1990 Plenum Publishing Corporation.
  • Digital Object Identifier (doi)

    Author List

  • Strong TV; Barker PE; Anderson SJ
  • Start Page

  • 341
  • End Page

  • 349
  • Volume

  • 16
  • Issue

  • 4