Chloride conductance expressed by delta F508 and other mutant CFTRs in Xenopus oocytes.

Academic Article

Abstract

  • The cystic fibrosis transmembrane conductance regulator (CFTR) is associated with expression of a chloride conductance that is defective in cystic fibrosis (CF). Xenopus oocytes injected with RNA coding for CFTR that contained mutations in the first nucleotide binding fold (NBF1) expressed chloride currents in response to raising adenosine 3',5'-monophosphate (cAMP) with forskolin and 3-isobutyl-1-methylxanthine (IBMX). The mutant CFTRs were less sensitive than wild-type CFTR to this activating stimulus, and the reduction in sensitivity correlated with the severity of cystic fibrosis in patients carrying the corresponding mutations. This demonstration provides the basis for detailed analyses of NBF1 function and suggests potential pharmacologic treatments for cystic fibrosis.
  • Authors

    Published In

  • Science  Journal
  • Keywords

  • 1-Methyl-3-isobutylxanthine, Animals, Chloride Channels, Chlorides, Cystic Fibrosis, Cystic Fibrosis Transmembrane Conductance Regulator, Genetic Variation, Genotype, Humans, Ion Channels, Membrane Potentials, Membrane Proteins, Microinjections, Mutation, Oocytes, RNA, Transcription, Genetic, Xenopus
  • Authorlist

  • Drumm ML; Wilkinson DJ; Smit LS; Worrell RT; Strong TV; Frizzell RA; Dawson DC; Collins FS
  • Start Page

  • 1797
  • End Page

  • 1799
  • Volume

  • 254
  • Issue

  • 5039