A DNA vaccine encoding genetic fusions of carcinoembryonic antigen (CEA) and granulocyte/macrophage colony-stimulating factor (GM-CSF).

Academic Article

Abstract

  • The anti-tumor immunologic effects of plasmid DNA vaccines encoding human carcinoembryonic antigen (CEA) fused to mouse granulocyte/macrophage colony-stimulating factor (GM-CSF) were examined. Immunization of C57BL/6 mice with the CEA-GMCSF fusion plasmids in a three injection, high-dose immunization schedule led to T cell and antibody responses specific for CEA. Mice injected with CEA-GMCSF fusion plasmids also developed IgG autoantibodies to GM-CSF. Tumor challenge with the CEA-expressing syngeneic mouse adenocarcinoma line, MC38-CEA-2, showed delayed tumor growth in mice immunized with the CEA-GMCSF fusion plasmids but complete protection in mice immunized with plasmid encoding CEA alone. In contrast, a single low-dose immunization with CEA-GMCSF fusion plasmids provided better tumor protection than low-dose CEA plasmid alone and resulted in lower titers of GM-CSF antibodies.
  • Published In

  • Vaccine  Journal
  • Keywords

  • Adenocarcinoma, Animals, Autoantibodies, Blotting, Western, Cancer Vaccines, Carcinoembryonic Antigen, Cell Line, Tumor, Cytokines, Dose-Response Relationship, Immunologic, Enzyme-Linked Immunosorbent Assay, Female, Granulocyte-Macrophage Colony-Stimulating Factor, Immunization, Immunoprecipitation, Mice, Mice, Inbred C57BL, Plasmids, Recombinant Fusion Proteins, Reverse Transcriptase Polymerase Chain Reaction, Survival Analysis, Vaccines, DNA
  • Digital Object Identifier (doi)

    Author List

  • Lima J; Jenkins C; Guerrero A; Triozzi PL; Shaw DR; Strong TV
  • Start Page

  • 1273
  • End Page

  • 1283
  • Volume

  • 23
  • Issue

  • 10