Pre-beta-HDL stimulates placental lactogen release from human trophoblast cells.

Academic Article

Abstract

  • To examine whether pre-beta-high-density lipoprotein (HDL) may be involved in regulation of human placental lactogen (hPL) release, pre-beta-HDL was isolated from term pregnancy serum, and the effect of purified pre-beta-HDL on hPL release from trophoblast cells was examined after 1 h of exposure. Pre-beta-HDL stimulated a dose-dependent increase in hPL release with half-maximal stimulation at a dose of 300-400 microgram/ml, which is within the normal physiological range during pregnancy. Analysis of pre-beta-HDL and alpha-HDL in serum from pregnant women at different stages of gestation (determined by Western blot analysis) indicated that the pre-beta-HDL-to-alpha-HDL ratio increased linearly after the 10th week of gestation (r = 0.88, P < 0.001), reaching a maximum sixfold greater than that of nonpregnant women. The increase in serum pre-beta-HDL during pregnancy paralleled that of plasma hPL concentrations (r = 0.93, P < 0.001). Two-dimensional electrophoresis indicated that the increase in pre-beta-HDL was due primarily to an increase in pre-beta1-HDL and pre-beta2-HDL, two of the three forms of pre-beta-HDL present in blood. These results suggest a role for pre-beta-HDL in the regulation of hPL expression during pregnancy.
  • Published In

    Keywords

  • Cells, Cultured, Dose-Response Relationship, Drug, Female, High-Density Lipoproteins, Pre-beta, Humans, Lipoproteins, HDL, Placental Lactogen, Pregnancy, Trophoblasts
  • Authorlist

  • Handwerger S; Datta G; Richardson B; Schmidt CM; Siddiqi T; Turzai L; Anantharamaiah GM
  • Start Page

  • E384
  • End Page

  • E389
  • Volume

  • 276
  • Issue

  • 2 Pt 1