Assessment of indocyanine green-labeled cetuximab to detect xenografted head and neck cancer cell lines.

Academic Article

Abstract

  • OBJECTIVE: The aim of this study is to determine the efficacy of indocyanine green (ICG) conjugated to antiepidermal growth factor receptor antibody (cetuximab) to image head and neck cancer. STUDY DESIGN: Mice (n = 3) were injected with unconjugated ICG and imaged at 100-second intervals for a total of 1000 seconds to assess imaging characteristics. Mice (n = 10) xenografted with SCC-1 cells were then systemically injected with cetuximab conjugated to indocyanine green and imaged over a 72-hour period. To assess the sensitivity and specificity, xenografted tumors underwent subtotal resections and then were assessed for residual disease by fluorescence stereomicroscopy and confirmed by histology. RESULTS: Tumors demonstrated excellent fluorescence 24 hours after injection of cetuximab-ICG. There was a direct relationship between fluorescence and the given dose of cetuximab-ICG. Following subtotal resection, we found fluorescence correlated with a sensitivity of 78.4% and specificity of 96%. CONCLUSIONS: This study provides evidence that supports further preclinical investigation of cetuximab in the evaluation of surgical margins, but linkage to ICG lacks the sensitivity for use in a clinical setting.
  • Keywords

  • Animals, Antibodies, Monoclonal, Antibodies, Monoclonal, Humanized, Cell Line, Tumor, Cetuximab, Head and Neck Neoplasms, Indocyanine Green, Male, Mice, Mice, SCID, Microscopy, Fluorescence, Neoplasm Transplantation, Sensitivity and Specificity, Transplantation, Heterologous
  • Digital Object Identifier (doi)

    Author List

  • Withrow KP; Gleysteen JP; Safavy A; Skipper J; Desmond RA; Zinn K; Rosenthal EL
  • Start Page

  • 729
  • End Page

  • 734
  • Volume

  • 137
  • Issue

  • 5