Systematic review of diagnostic tests for vaginal trichomoniasis.

Academic Article

Abstract

  • OBJECTIVE: To review critically and to summarize the evidence of diagnostic tests and culture media for the diagnosis of Trichomonas vaginitis. METHODS: We performed a systematic review of literature indexed in MEDLINE of studies that used Trichomonas culture as the reference standard (9,882 patients, 35 studies). Level I studies (5,047 patients, 13 studies) fulfilled at least two of three criteria: 1) consecutive patients were evaluated prospectively, 2) decision to culture was not influenced by test results, and 3) there was independent and blind comparison to culture. RESULTS: The sensitivity of the polymerase chain reaction technique (PCR) was 95% (95% CI 91% to 99%), and the specificity was 98% (95% CI 96% to 100%). One study was classified as Level I evidence (52 patients). The sensitivity of the enzyme-linked immunosorbent assay was 82% (95% CI 74% to 90%), and the specificity was 73% (95% CI 35% to 100%). The sensitivity of the direct fluorescence antibody was 85% (95% CI 79% to 90%), and the specificity was 99% (95% CI 98% to 100%). Sensitivities of culture media were 95% for Diamond's, 96% for Hollander, and 95% for CPLM. CONCLUSIONS: The sensitivity and specificity of tests to diagnose trichomoniasis vary widely.
  • Authors

    Keywords

  • Animals, Culture Media, Enzyme-Linked Immunosorbent Assay, Female, Fluorescent Antibody Technique, Direct, Humans, MEDLINE, Polymerase Chain Reaction, Prospective Studies, Sensitivity and Specificity, Trichomonas Vaginitis, Trichomonas vaginalis
  • Digital Object Identifier (doi)

    Author List

  • Patel SR; Wiese W; Patel SC; Ohl C; Byrd JC; Estrada CA
  • Start Page

  • 248
  • End Page

  • 257
  • Volume

  • 8
  • Issue

  • 5-6