Poliovirus replicons encoding the B subunit of Helicobacter pylori urease protect mice against H. pylori infection.

Academic Article

Abstract

  • We developed a novel vaccine for Helicobacter pylori based on a poliovirus vector in which capsid genes were replaced with the gene for the B subunit of H. pylori urease (UreB). Mice were vaccinated with UreB or control (L1) replicon and challenged with H. pylori. Twenty percent of mice vaccinated prophylactically with UreB, but 80% vaccinated with L1, and then challenged with H. pylori became infected (P = 0.003). Seventy-three percent of mice with established H. pylori infection vaccinated therapeutically with UreB replicon cleared their infection compared to 33% vaccinated with L1 (P = 0.067). In therapeutically vaccinated mice with residual infection, UreB-vaccinated animals had fewer H. pylori than L1-vaccinated mice (P < 0.05). Anti-urease antibody titres in prophylactically, but not therapeutically, vaccinated mice were markedly higher in animals that received UreB versus L1 replicon (P = 0.01). Vaccination with poliovirus vector containing the gene for the B subunit of H. pylori urease provides significant prophylactic and strong therapeutic protection against H. pylori in mice.
  • Published In

  • Vaccine  Journal
  • Keywords

  • Animals, Bacterial Vaccines, HeLa Cells, Helicobacter Infections, Helicobacter pylori, Humans, Mice, Mice, Inbred C57BL, Poliovirus, Replicon, Urease, Vaccines, Subunit
  • Digital Object Identifier (doi)

    Author List

  • Smythies LE; Novak MJ; Waites KB; Lindsey JR; Morrow CD; Smith PD
  • Start Page

  • 901
  • End Page

  • 909
  • Volume

  • 23
  • Issue

  • 7