Characterization of peptide YY receptors in rabbit colonic mucosa

Academic Article

Abstract

  • Quantitative receptor autoradiography localized a high-affinity binding site for 125I-peptide YY to the mucosa in rabbit distal colon. Scatchard binding analysis revealed a single-affinity binding site (KD = 0.29 nM) with binding specificity similar to other peptide YY-preferring receptors (peptide YY ≥ neuropeptide Y > pancreatic polypeptide). Radioligand binding studies using colonic mucosal membranes confirmed high-affinity peptide YY binding sites (KD = 0.26 nM) with time, temperature, and protein dependence, as well as saturability characteristic of receptor-ligand binding. Selective peptide analogues showed a subpopulation of these 125I-peptide YY binding sites to resemble the Y1-type neuropeptide Y receptor. Peptide YY may exert local antisecretory effects on the colonic epithelium via these binding sites. © 1993.
  • Published In

  • Peptides  Journal
  • Digital Object Identifier (doi)

    Author List

  • Mannon PJ; Hernandez EJ; Mervin SJ; Vigna SR; Taylor IL
  • Start Page

  • 567
  • End Page

  • 572
  • Volume

  • 14
  • Issue

  • 3