Human astrocytoma cells express a unique chloride current

Academic Article

Abstract

  • Human astrocytoma cells were studied using whole-cell patch-clamp recording. Voltage-dependent outwardly-rectifying anion currents were identified in primary cultures of six freshly resected human brain tumors and in seven established anaplastic astrocytoma/glioblastoma cell lines (U251MG, CH235MG, U373MG, U105MG, D54MG, SK-MG-1, and STTG1). Anion currents were not observed in normal, non-neoplastic glial cells, nor in human tumor-derived cells of non-glial origin (melanoma, breast cancer, neuroblastoma, rhabdomyosarcoma). Currents activated at potentials >50 mV and showed large transients upon termination of voltage steps. Currents reversed at the predicted equilibrium potential for chloride ions and could also be recorded when Cl was replaced by F , Br or I . Currents were inhibited by the Cl channel blockers chlorotoxin, DIDS, and DNDS. These Cl currents may play a role in the growth control of astrocytoma cells. - - - - - -
  • Published In

  • NeuroReport  Journal
  • Digital Object Identifier (doi)

    Author List

  • Ullrich N; Gillespie GY; Sontheimer H
  • Start Page

  • 1020
  • End Page

  • 1024
  • Volume

  • 7
  • Issue

  • 5