Increased expression of thymidylate synthetase (TS), ubiquitin specific protease 10 (USP10) and survivin is associated with poor survival in glioblastoma multiforme (GBM).

Academic Article

Abstract

  • BACKGROUND: The limited success of empirically designed treatment paradigms for patients diagnosed with glioblastoma multiforme (GBM) emphasizes the need for rationally designed treatment strategies based on the molecular profile of tumor samples and their correlation to clinical parameters. METHODS: In the current study, we utilize a novel real-time quantitative low density array (RTQ-LDA) to identify differentially expressed genes in de novo GBM tissues obtained from patients with distinctly different clinical outcomes. Total RNA was isolated from a cohort of 21 GBM specimens obtained from patients with either good (long-term survival (LTS) >36 months post surgery, n = 8) or poor (died of the disease (DOD) <24 months post surgery, n = 13) prognosis. Non-neoplastic brain tissue (n = 5) was obtained from patients who underwent surgery for refractory epilepsy. Demographic data was assessed for correlation with survival using Cox proportional hazards models. Sufficient RNA was available to use RTQ-LDA to quantify the expression of 93 independent genes in 5 LTS, 4 DOD, and 5 non-neoplastic brain samples. The eight differentially expressed genes identified by RTQ-LDA in LTS versus DOD (P
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    Keywords

  • Adolescent, Adult, Age Factors, Aged, Biomarkers, Tumor, Brain Neoplasms, Child, Disease-Free Survival, Endopeptidases, Female, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Glioblastoma, Humans, Inhibitor of Apoptosis Proteins, Male, Microtubule-Associated Proteins, Middle Aged, Neoplasm Proteins, RNA, Retrospective Studies, Statistics, Nonparametric, Survivin, Thymidylate Synthase, Ubiquitin Thiolesterase
  • Digital Object Identifier (doi)

    Author List

  • Grunda JM; Nabors LB; Palmer CA; Chhieng DC; Steg A; Mikkelsen T; Diasio RB; Zhang K; Allison D; Grizzle WE
  • Start Page

  • 261
  • End Page

  • 274
  • Volume

  • 80
  • Issue

  • 3