In this paper, the behavior of a micron-scale fluid droplet on a heterogeneous surface is investigated using a two-phase lattice Boltzmann method (LBM). The two-phase LBM permits the simulation of the time dependent three-dimensional motion of a liquid droplet on solid surface patterned with hydrophobic and hydrophilic strips. A nearest-neighbor molecular interaction force is used to model the adhesive forces between the fluid and solid walls. The solid heterogeneous wall is a uniform hydrophilic substrate painted with hydrophobic strips. The model is validated by demonstrating the consistency of the simulation results with an exact solution for capillary rise and through qualitative comparison of computed dynamic contact line behavior with experimentally measured surface properties and observed surface shapes of a droplet on a heterogeneous surface. The dependence of spreading behavior on wettability, the width of hydrophobic strip, initial location of the droplet relative to the strips, and gravity is investigated. A decrease in contact angle of the liquid on a hydrophilic surface may lead to breakup of the droplet for certain substrate patterns. The simulations suggest that the present lattice Boltzmann (LB) model can be used as a reliable way to study fluidic control on heterogeneous surfaces and other wetting related subjects. © Springer-Verlag 2006.