The Honeywell In-Space Accelerometer system (HISA) collected data in the mid-deck area of the Shuttle Columbia during the flight of STS-32, January 1990. The resulting data were to be used to investigate the response of crystal microstructure to different types of residual acceleration. The HISA, using Sundstrand QA-2000 sensors, is designed to detect and record transient and oscillatory accelerations. The sampling and electronics package stored averaged accelerations over two sampling periods; two sampling rates were available: 1 Hz and 50 Hz. Analysis of the HISA data followed the CMMR Acceleration Data Processing Guide, considering in-house computer modelling of a float-zone indium crystal growth experiment. Characteristic examples of HISA data showing the response to the primary reaction control system (PRCS), Orbiter Maneuvering System (OMS) operations and crew treadmill activity are presented. Various orbiter structural modes are excited by these and other activities. Such modes are in the 1 Hz to 20 Hz range. Of particular note is the distinct frequency of crew footfalls during exercise periods and the resultant excitation of orbiter frequencies. Acceleration vector magnitudes ranged from 10-4 g to >10-2 g. Acceleration values recorded at the crystal growth site exceeded time domain tolerance limits in approximately 35% of the data, especially during PRCS firings, OMS burns, and treadmill exercise. Frequency domain limits, however, were not exceeded.