Context: Pediatric studies have shown that aerobic exercise reduces metabolic risk, but dose-response information is not available. Objectives: To test the effect of different doses of aerobic training on insulin resistance, fatness, visceral fat, and fitness in overweight, sedentary children and to test moderation by sex and race. Design, Setting, and Participants: Randomized controlled efficacy trial conducted from 2003 through 2007 in which 222 overweight or obese sedentary children (mean age, 9.4 years; 42% male; 58% black) were recruited from 15 public schools in the Augusta, Georgia, area. Intervention: Children were randomly assigned to low-dose (20 min/d; n=71) or high-dose (40 min/d; n=73) aerobic training (5 d/wk; mean duration, 13 [SD, 1.6] weeks) or a control condition (usual physical activity; n=78). Main Outcome Measures: The prespecified primary outcomes were postintervention type 2 diabetes risk assessed by insulin area under the curve (AUC) from an oral glucose tolerance test, aerobic fitness (peak oxygen consumption [V̇O 2]), percent body fat via dual-energy x-ray absorptiometry, and visceral fat via magnetic resonance, analyzed by intention to treat. Results: The study had 94% retention (n=209). Most children (85%) were obese. At baseline, mean body mass index was 26 (SD, 4.4). Reductions in insulin AUC were larger in the high-dose group (adjusted mean difference, -3.56 [95% CI, -6.26 to -0.85] ×103 μU/mL; P=.01) and the low-dose group (adjusted mean difference, -2.96 [95% CI, -5.69 to -0.22] ×103 μU/mL; P=.03) than the control group. Dose-response trends were also observed for body fat (adjusted mean difference, -1.4% [95% CI, -2.2% to -0.7%]; P<.001 and -0.8% [95% CI, -1.6% to -0.07%]; P=.03) and visceral fat (adjusted mean difference, -3.9 cm3 [95% CI, -6.0 to -1.7 cm3]; P<.001 and -2.8 cm3 [95% CI, -4.9 to -0.6 cm3]; P=.01) in the high- and low-dose vs control groups, respectively. Effects in the high- and low-dose groups vs control were similar for fitness (adjusted mean difference in peak V̇O2, 2.4 [95% CI, 0.4-4.5] mL/kg/min; P=.02 and 2.4 [95% CI, 0.3-4.5] mL/kg/min; P=.03, respectively). High- vs low-dose group effects were similar for these outcomes. There was no moderation by sex or race. Conclusion: In this trial, after 13 weeks, 20 or 40 min/d of aerobic training improved fitness and demonstrated dose-response benefits for insulin resistance and general and visceral adiposity in sedentary overweight or obese children, regardless of sex or race. Trial Registration clinicaltrials.gov Identifier: NCT00108901. ©2012 American Medical Association. All rights reserved.