Preparation and use of a photoactivatable glucose-6-phosphate analogue

Academic Article

Abstract

  • A benzophenone-containing derivative of glucose-6-phosphate, 6-[(3-([2,3-3H2]-p-benzoyl dihydrocinnamidoylpropyl-1-oxy)phosphoryl]-D-glucopyranose ([3H]BZDC-Glc-6-P) was synthesized and employed to photoaffinity label proteins on intact rat liver microsomes. The use of a non-photoactivatable, UV-transparent desoxy analogue of BZDC, named p-benzyldihydrocinnamoyl (BnDC), is introduced as a general method to achieve competition when hydrophilic ligands are modified with hydrophobic photophores. (C) 2000 Elsevier Science Ltd. All rights reserved.
  • Digital Object Identifier (doi)

    Author List

  • Peng J; Chen PY; Marchase RB; Prestwich GD
  • Start Page

  • 535
  • End Page

  • 539
  • Volume

  • 10
  • Issue

  • 6