Separation of ectoderm and endoderm from sea urchin pluteus larvae and demonstration of germ layer-specific antigens

Academic Article


  • A simple method is described for the separation of ectoderm from endoderm in sea urchin pluteus larvae. The separation, by sequential treatments with glycine-EDTA, permits an examination of the basement membrane beneath the ectoderm layer and of a fibrous layer beneath the basement membrane. In a serological study the two germ layers are shown to differ with respect to cell surface antigen composition. Further, the basement membrane is shown to contain ectoderm antigens. © 1979 Academic Press, Inc. All rights reserved.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • McClay DR; Marchase RB
  • Start Page

  • 289
  • End Page

  • 296
  • Volume

  • 71
  • Issue

  • 2