The purpose of the investigation was to determine the validity of a portable vertical accelerometer and a Large-Scale Integrated Motor Activity Monitor (LSI) for estimating energy expenditure in riding a bicycle at various velocities. Instrument placement was either at the knee or ankle. Energy consumption, i.e. oxygen consumption (VO2) was determined during bicycle rides after steady state metabolism was reached. Standard errors of estimate were used to express the accuracy of estimating VO2 from accelerometer or LSI recordings. The reliability of the vertical accelerometer was found to be satisfactory. The vertical accelerometer was also effective for estimating VO2 in bicycling (standard errors of estimate = 3.3 to 4.4 ml.kg-1.min-1). The accuracy of the LSI was not as good; the standard errors of estimate being = 5.9 to 8.5 ml.kg-1.min-1.