Investigation of the hyper-reflective inner/outer segment band in optical coherence tomography of living frog retina.

Academic Article


  • This study is to test anatomic correlates, including connecting cilium (CC) and inner segment (IS) ellipsoid, to the hyper-reflective band visualized by optical coherence tomography (OCT) and commonly attributed to the photoreceptor inner/outer segment (IS/OS) junction. A line-scan OCT (LS-OCT) was constructed to achieve sub-cellular resolution (lateral: ≈ 2 μm; axial: ≈ 4 μm) of excised living frog retinas. An electro-optic phase modulator was employed for rapid and vibration-free phase modulation. Comparison of normalized distance measurements between LS-OCT images and histological images revealed that the dominant source of the signal reported as the IS/OS OCT band actually originates from the IS.
  • Published In


  • Algorithms, Animals, Electrophysiology, Equipment Design, Light, Optics and Photonics, Photoreceptor Cells, Vertebrate, Ranidae, Reproducibility of Results, Retina, Retinal Photoreceptor Cell Outer Segment, Signal Processing, Computer-Assisted, Tomography, Optical Coherence
  • Digital Object Identifier (doi)

    Author List

  • Lu R-W; Curcio CA; Zhang Y; Zhang Q-X; Pittler SJ; Deretic D; Yao X-C
  • Start Page

  • 060504
  • Volume

  • 17
  • Issue

  • 6