In this chapter, the authors discuss challenges and decision-making in the process of conducting quality mixed methods research in higher education, and offer the lessons learned from their experiences. The chapter begins with a discussion of quality and the ways of establishing quality in mixed methods studies. Two examples of studies are used to illustrate the issues involved in addressing quality in conducting mixed methods studies in different higher education settings. The first example discusses the challenges associated with establishing the quality of metainferences in a mixed methods (quantitative to qualitative) design that was used in two studies of students' engagement and persistence in pursuing graduate degrees online in the United States. The second example presents the methodological steps and criteria for evaluating the quality of a multilevel mixed design study to explain quality management in Greek higher education. The authors also reflect on how researchers can become active participants in the co-construction of quality in mixed methods research. © 2013 by Emerald Group Publishing Limited All rights of reproduction in any form reserved.