A DNA gyrase-binding site at the center of the bacteriophage Mu genome is required for efficient replicative transposition

Academic Article

Abstract

  • We have discovered a centrally located site that is required for efficient replication of bacteriophage Mu DNA and identified it as a strong DNA gyrase-binding site. Incubation of Mu DNA with gyrase and enoxacin revealed a cleavage site 18.1 kilobases from the left end of the 37.2-kilobase genome. Two observations indicate a role for the site in Mu replication: mutants of Mu, able to grow on an Escherichia coli gyrB host that does not allow growth of wild-type Mu, were found to possess single-base changes resulting in more efficient gyrase binding and cleavage at the site. Introduction of a 147-base-pair deletion that eliminated the site from a prophage inhibited the onset of Mu replication for > 1 hr after induction.
  • Authors

    Digital Object Identifier (doi)

    Author List

  • Pato ML; Howe MM; Higgins NP
  • Start Page

  • 8716
  • End Page

  • 8720
  • Volume

  • 87
  • Issue

  • 22