Needle contamination in the setting of intravitreal injections.

Academic Article


  • BACKGROUND/PURPOSE: To evaluate the risk of intravitreal needle contamination through speaking versus breathing in an office setting. METHODS: This was a prospective sampling assay. Participants held a sterile 30-gauge half-inch needle 25 cm from their mouth for 30 seconds under 2 conditions: (1) while speaking and (2) while breathing silently. Needles were then cultured and assayed after 6 days of incubation. Absolute colony-forming units were compared between conditions and against control sterile needles and oral swab cultures. RESULTS: Ten physicians were sampled with 15 samples per physician. Participants grew an average of 0.21 colonies (median = 1 CFU) from their talking samples and 0.07 colonies (median = 1 CFU) from their silent breathing samples. Oral swab plates grew an average of 373.4 colonies. None of the control needle plates grew colony-forming units. A nominal regression analysis showed no significant difference between talking and silent samples (P = 0.457). CONCLUSION: No significant difference in needle contamination was found between talking and breathing. Compared with oral swab plates, a significant difference exists between the amount of flora colonizing the oropharynx and that which was found on the needle cultures (P < 0.0001). These findings suggest that speaking versus remaining silent makes no difference in regard to needle contamination with oral flora during intravitreal injection.
  • Authors

    Published In

  • RETINA  Journal
  • Keywords

  • Bacteria, Colony Count, Microbial, Equipment Contamination, Humans, Intravitreal Injections, Mouth Mucosa, Needles, Prospective Studies, Respiration, Risk Factors, Speech
  • Digital Object Identifier (doi)

    Author List

  • Friedman DA; Lindquist TP; Mason JO; McGwin G
  • Start Page

  • 929
  • End Page

  • 934
  • Volume

  • 34
  • Issue

  • 5