Human glioblastoma cells produce 77 amino acid interleukin-8 (IL-877)

Academic Article

Abstract

  • The production of interleukin 8 (IL-8), a neutrophil chemotactic factor, and its amino acid sequence were examined in glioblastoma cell lines in vitro. Neutrophil chemotactic activity was demonstrated in 9 conditioned media of 15 human glioblastoma cell lines. Tumor necrosis factor (TNF)-α stimulated secretion of the activity in 7 lines and induced secretion in 4 other lines. ELISA quantification disclosed that the conditioned media contained interleukin 8 (IL-8) in an amount equivalent to the chemotactic activity. The IL-8 secretion increased with the stimulation by TNF-α. Northern blot analysis and the RT-PCR method confirmed expression of mRNA in the glioblastoma cells and its augmentation by TNF-α and/or IL-β. Reversed-phase HPLC following ion-exchange chromatography revealed that the chemotactic activity was a single peptide, which was determined to be IL-8 by the retention time and ELISA. Furthermore, amino acid analysis disclosed that a major part of the glioblastoma-cell derived IL-8 peptide was 77 amino acid IL-8 (IL-877; with the N-terminal sequence AVLPRSAKELRCQCI-). © 1993 Kluwer Academic Publishers.
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    Digital Object Identifier (doi)

    Author List

  • Tada M; Suzuki K; Yamakawa Y; Sawamura Y; Sakuma S; Abe H; van Meir E; de Tribolet N
  • Start Page

  • 25
  • End Page

  • 34
  • Volume

  • 16
  • Issue

  • 1