Gene transfer into mammalian central nervous system using herpes virus vectors: Extended expression of bacterial lacZ in neurons using the neuron-specific enolase promoter

Academic Article

Abstract

  • A herpes simplex virus (HSV) vector in which the mammalian promoter for neuron-specific enolase (NSE) controls expression of a marker gene was analyzed for its ability to drive expression of this foreign gene in culture and in vivo. In cultured cells, the vector appeared to give neuron-specific expression. Introduction of 106 pfu of the virus into the adult rat caudate nucleus by stereotactic injection was not toxic to the animals and yielded β-galactosidase (β-gal)-positive neurons for at least 30 days after viral inoculation. This recombinant herpes virus vector is the first described to use a mammalian promoter to yield extended expression of a foreign gene product in the adult mammalian central nervous system (CNS).
  • Authors

    Published In

  • Human Gene Therapy  Journal
  • Digital Object Identifier (doi)

    Author List

  • Andersen JK; Garber DA; Meaney CA; Breakefield XO
  • Start Page

  • 487
  • End Page

  • 499
  • Volume

  • 3
  • Issue

  • 5