© 2019 Elsevier B.V. Gelatin is a biocompatible polymer that is widely used in tissue engineering. Nowadays, nanofibrous scaffolds are produced mainly by the direct voltage spinning method which, however, does not currently have the same production capacity as the newly developed alternating current (AC) spinning technology. Using AC spinning, nanofibrous layers made from a 20% concentration of gelatin in a solvent system containing acetic acid, distilled water, and ethanol were spun. A solution containing minimal acetic acid which could still be spun optimally was searched for spinning. The produced gelatin nanofibrous layers were crosslinked and, under various conditions, subjected to mechanical testing.