Gene bookmarking accelerates the kinetics of post-mitotic transcriptional re-activation

Academic Article

Abstract

  • Although transmission of the gene expression program from mother to daughter cells has been suggested to be mediated by gene bookmarking, the precise mechanism by which bookmarking mediates post-mitotic transcriptional re-activation has been unclear. Here, we used a real-time gene expression system to quantitatively demonstrate that transcriptional activation of the same genetic locus occurs with a significantly more rapid kinetics in post-mitotic cells versus interphase cells. RNA polymerase II large subunit (Pol II) and bromodomain protein 4 (BRD4) were recruited to the locus in a different sequential order on interphase initiation versus post-mitotic re-activation resulting from the recognition by BRD4 of increased levels of histone H4 Lys 5 acetylation (H4K5ac) on the previously activated locus. BRD4 accelerated the dynamics of messenger RNA synthesis by de-compacting chromatin and hence facilitating transcriptional re-activation. Using a real-time quantitative approach, we identified differences in the kinetics of transcriptional activation between interphase and post-mitotic cells that are mediated by a chromatin-based epigenetic mechanism. © 2011 Macmillan Publishers Limited. All rights reserved.
  • Authors

    Published In

    Digital Object Identifier (doi)

    Pubmed Id

  • 14452057
  • Author List

  • Zhao R; Nakamura T; Fu Y; Lazar Z; Spector DL
  • Start Page

  • 1295
  • End Page

  • 1304
  • Volume

  • 13
  • Issue

  • 11