bcl-2 gene therapy exacerbates excitotoxicity

Academic Article


  • The protooncogene bcl-2 can block neuronal death from both naturally occurring apoptosis and exogenous insults, bcl-2 is therefore a promising candidate for the prevention of excitotoxic neuronal death. Using an adeno- associated viral vector, we delivered the bcl-2 gene to the ganglion cell layer of the rat eye. We hypothesized that infection with bcl-2 would protect ganglion cells against excitotoxic cell death. However, retinal infection with bcl-2 increased ganglion cell susceptibility to both axonal injury and intravitreal NMDA. Our study-intended to explore the possibility of bcl-2 transduction as an in vivo therapeutic approach-revealed a deleterious effect of bcl-2 transduction.
  • Authors

    Published In

  • Human Gene Therapy  Journal
  • Digital Object Identifier (doi)

    Pubmed Id

  • 1951446
  • Author List

  • Simon PD; Vorwerk CK; Mansukani SS; Chen SJ; Wilson JM; Zurakowski D; Bennett J; Dreyer EB
  • Start Page

  • 1715
  • End Page

  • 1720
  • Volume

  • 10
  • Issue

  • 10