A radiation hybrid map of the region on human chromosome 22 containing the neurofibromatosis type 2 locus

Academic Article

Abstract

  • We describe a high-resolution radiation hybrid map of the region on human chromosome 22 containing the neurofibromatosis type 2 (NF2) gene. Eighty-five hamster-human somatic cell hybrids generated by X-irradiation and cell fusion were used to generate the radiation hybrid map. The presence or absence of 18 human chromosome 22-specific markers was determined in each hybrid by using Southern blot hybridization. Sixteen of the 18 markers were distinguishable by X-ray breakage in the radiation hybrids. Analysis of these data using two different mathematical models and two different statistical methods resulted in a single framework map consisting of 8 markers ordered with odds greater than 1000:1. The remaining nonframework markers were all localized to regions consisting of two adjoining intervals on the framework map with odds greater than 1000:1. Based on the RH map, the NF2 region of chromosome 22, defined by the flanking markers D22S1 and D22S28, is estimated to span a physical distance of approximately 6 Mb and is the most likely location for 9 of the 18 markers studied: D22S33, D22S41, D22S42, D22S46, D22S56, LIF, D22S37, D22S44, and D22S15. © 1992 Academic Press, Inc. All rights reserved.
  • Published In

  • Genomics  Journal
  • Digital Object Identifier (doi)

    Author List

  • Frazer KA; Boehnke M; Budarf ML; Wolff RK; Emanuel BS; Myers RM; Cox DR
  • Start Page

  • 574
  • End Page

  • 584
  • Volume

  • 14
  • Issue

  • 3