An integrated software system for analyzing ChIP-chip and ChIP-seq data

Academic Article

Abstract

  • We present CisGenome, a software system for analyzing genome-wide chromatin immunoprecipitation (ChIP) data. CisGenome is designed to meet all basic needs of ChIP data analyses, including visualization, data normalization, peak detection, false discovery rate computation, gene-peak association, and sequence and motif analysis. In addition to implementing previously published ChIP-microarray (ChIP-chip) analysis methods, the software contains statistical methods designed specifically for ChlP sequencing (ChIP-seq) data obtained by coupling ChIP with massively parallel sequencing. The modular design of CisGenome enables it to support interactive analyses through a graphic user interface as well as customized batch-mode computation for advanced data mining. A built-in browser allows visualization of array images, signals, gene structure, conservation, and DNA sequence and motif information. We demonstrate the use of these tools by a comparative analysis of ChIP-chip and ChIP-seq data for the transcription factor NRSF/REST, a study of ChIP-seq analysis with or without a negative control sample, and an analysis of a new motif in Nanog- and Sox2-binding regions. © 2008 Nature Publishing Group.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Ji H; Jiang H; Ma W; Johnson DS; Myers RM; Wong WH
  • Start Page

  • 1293
  • End Page

  • 1300
  • Volume

  • 26
  • Issue

  • 11