Rapid development of nucleic acid diagnostics

Academic Article

Abstract

  • There has been a significant increase, fueled by technologies from the human genome project, in the availability of nucleic acid sequence information for viruses and bacteria. This paper presents a computer-assisted process that begins with nucleic acid sequence information and produces highly specific pathogen signatures. When combined with instrumentation using the polymerase chain reaction, the resulting diagnostics are both specific and sensitive. The computational and engineering aspects of converting raw sequence data into pathogen-specific and instrument-ready assays are presented. Examples and data are presented for specific pathogens, including foot-and-mouth disease virus and the human immunodeficiency virus. © 2002 IEEE.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Fitch JP; Gardner SN; Kuczmarski TA; Kurtz S; Myers R; Ott LL; Slezak TR; Vitalis EA; Zemla AT; Mccready PM
  • Start Page

  • 1708
  • End Page

  • 1720
  • Volume

  • 90
  • Issue

  • 11