Segregation of the Huntington disease region of human chromosome 4 in a somatic cell hybrid

Academic Article

Abstract

  • We have developed an X-irradiation: cell fusion procedure that segregates segments of human chromosomes lacking selectable markers and have used this approach to construct somatic cell hybrids retaining fragments of human chromosome 4 as the only human material. To identify hybrids retaining a small chromosomal fragment in the region of the Huntington disease (HD) gene, we used Southern blot analysis to screen 72 hybrid lines for the presence or absence of seven chromosome 4 single-copy loci. These data, combined with in situ hybridization experiments, identified three hybrids of interest. One of these cell lines, C25, stably retains a 10,000- to 20,000-kb fragment of distal 4p in the vicinity of the HD gene, translocated to a hamster chromosome. Field-inversion gel electrophoresis revealed no evidence of rearrangements in the human DNA present in C25. In combination with similar radiation hybrids, C25 is a valuable tool for isolating DNA probes near the HD gene. © 1989.
  • Published In

  • Genomics  Journal
  • Digital Object Identifier (doi)

    Author List

  • Cox DR; Pritchard CA; Uglum E; Casher D; Kobori J; Myers RM
  • Start Page

  • 397
  • End Page

  • 407
  • Volume

  • 4
  • Issue

  • 3