Exon trapping: A genetic screen to identify candidate transcribed sequences in cloned mammalian genomic DNA

Academic Article

Abstract

  • Identification and recovery of transcribed sequences from cloned mammalian genomic DNA remains an important problem in isolating genes on the basis of their chromosomal location. We have developed a strategy that facilitates the recovery of exons from random pieces of cloned genomic DNA. The basis of this 'exon trapping' strategy is that, during a retroviral life cycle, genomic sequences of nonviral origin are correctly spliced and may be recovered as a cDNA copy of the introduced segment. By using this genetic assay for cis-acting sequences required for RNA splicing, we have screened ≃20 kilobase pairs of cloned genomic DNA and have recovered all four predicted exons.
  • Digital Object Identifier (doi)

    Author List

  • Duyk GM; Kim S; Myers RM; Cox DR
  • Start Page

  • 8995
  • End Page

  • 8999
  • Volume

  • 87
  • Issue

  • 22