α 1G T-type calcium channel determines the angiogenic potential of pulmonary microvascular endothelial cells

Academic Article

Abstract

  • Pulmonary microvascular endothelial cells (PMVECs) display a rapid angioproliferative phenotype, essential for maintaining homeostasis in steady-state and promoting vascular repair after injury. Although it has long been established that endothelial cytosolic Ca 2+ ([Ca 2+ ] i ) transients are required for proliferation and angiogenesis, mechanisms underlying such regulation and the transmembrane channels mediating the relevant [Ca 2+ ] i transients remain incompletely understood. In the present study, the functional role of the microvascular endothelial site-specific α 1G T-type Ca 2+ channel in angiogenesis was examined. PMVECs intrinsically possess an in vitro angiogenic “network formation” capacity. Depleting extracellular Ca 2+ abolishes network formation, whereas blockade of vascular endothelial growth factor receptor or nitric oxide synthase has little or no effect, suggesting that the network formation is a [Ca 2+ ] i -dependent process. Blockade of the T-type Ca 2+ channel or silencing of α 1G , the only voltage-gated Ca 2+ channel subtype expressed in PMVECs, disrupts network formation. In contrast, blockade of canonical transient receptor potential (TRP) isoform 4 or TRP vanilloid 4, two other Ca 2+ permeable channels expressed in PMVECs, has no effect on network formation. T-type Ca 2+ channel blockade also reduces proliferation, cell-matrix adhesion, and migration, three major components of angiogenesis in PMVECs. An in vivo study demonstrated that the mice lacking α 1G exhibited a profoundly impaired postinjury cell proliferation in the lungs following lipopolysaccharide challenge. Mechanistically, T-type Ca 2+ channel blockade reduces Akt phosphorylation in a dose-dependent manner. Blockade of Akt or its upstream activator, phos-phatidylinositol-3-kinase (PI3K), also impairs network formation. Altogether, these findings suggest a novel functional role for the α 1G T-type Ca 2+ channel to promote the cell’s angiogenic potential via a PI3K-Akt signaling pathway.
  • Authors

    Digital Object Identifier (doi)

    Author List

  • Zheng Z; Chen H; Xie P; Dickerson CA; King JAC; Alexeyev MF; Wu S
  • Start Page

  • C353
  • End Page

  • C364
  • Volume

  • 316
  • Issue

  • 3