Structural mutations of the T cell receptor ζ chain and its role in T cell activation

Academic Article

Abstract

  • T cell hybridomas that express ζζ, but not ζη, dimers in their T cell receptors (TCRs) produce interleukin-2 (IL-2) and undergo an inhibition of spontaneous growth when activated by antigen, antibodies to the receptor, or antibodies to Thy-1. Hybridomas without ζ and η were reconstituted with mutated; chains. Cytoplasmic truncations of up to 40% of the ζ molecule reconstituted normal surface assembly of TCRs, but antigen-induced IL-2 secretion and growth inhibition were lost. In contrast, crosslinking antibodies to the TCR activated these cells. A point mutation conferred the same signaling phenotype as did the truncations and caused defective antigen-induced tyrosine kinase activation. Thus, ζ allows the binding of antigen/major histocompatibility complex (MHC) to ap to effect TCR signaling.
  • Published In

  • Science  Journal
  • Digital Object Identifier (doi)

    Author List

  • Frank SJ; Niklinska BB; Orloff DG; Merćep M; Ashwell JD; Klausner RD
  • Start Page

  • 174
  • End Page

  • 177
  • Volume

  • 249
  • Issue

  • 4965