Robotic real-time near infrared targeted fluorescence imaging in a murine model of prostate cancer: A feasibility study

Academic Article

Abstract

  • Objective: To evaluate the detection of near-infrared fluorescence from prostate tumors stained with a prostate-specific membrane antigen (PSMA)-targeted tracer developed in our institution with a novel robotic imaging system. Methods: Prostate cancer cell lines PC3-pip (PSMA positive) and PC3-flu (PSMA negative) were implanted subcutaneously into 6 immunodeficient mice. When tumors reached 5 mm, a PSMA-targeted fluorescent conjugate was injected intravenously. The first 3 mice underwent near-infrared imaging immediately and hourly up to 4 hours after injection to determine the time necessary to obtain peak fluorescence and were killed. The last 3 mice were imaged once preoperatively and were euthanized 120 minutes later. Excision of the tumors was performed by using a novel robotic imaging system to detect near-infrared fluorescence in real time. Specimens were submitted for pathology. Results: In the first 3 mice, we found 120 minutes as the time needed to observe peak fluorescence from the PSMA-positive tumors. We identified discrete near-infrared fluorescence from 2 of 3 PSMA-positive tumors with the robotic imaging system. Surgical margins were negative for all excised specimens except for one PSMA-negative tumor. Conclusions: Real-time near-infrared fluorescence imaging of prostate cancer is feasible with a novel robotic imaging system. Further research is needed to optimize the signal intensity detectable from prostate cancer with our tracer. Toxicologic studies are needed before its clinical use. © 2013 Elsevier Inc.
  • Published In

  • Urology  Journal
  • Digital Object Identifier (doi)

    Pubmed Id

  • 19525392
  • Author List

  • Laydner H; Huang SS; Heston WD; Autorino R; Wang X; Harsch KM; Magi-Galluzzi C; Isac W; Khanna R; Hu B
  • Start Page

  • 451
  • End Page

  • 457
  • Volume

  • 81
  • Issue

  • 2