Enzymes of ammonium assimilation in Streptomyces avermitilis

Academic Article

Abstract

  • Glutamine synthetase (GS), glutamate synthase (GOGAT), glutamate dehydrogenase (GDH), alanine dehydrogenase (ADH) and alanine aminotransferase (GPT) were detected in the cell-free homogenate of Streptomyces avermitilis grown in a defined medium containing ammonium sulfate as the only nitrogen source. At an initial NH4+ concentration of 7.5 mmol/L, high activities of GS, GOGAT and GDH were found while that of ADH was low. The ADH activity was markedly increased at initially millimolar NH4+ concentrations. In some characteristics of its NH4+-assimilating system (e.g. control of some enzyme activities, the NADPH specificity of GOGAT, the presence of alanine aminotransferase), S. avermitilis differs from other known streptomycetes. © 1992, Folia Microbiologica. All rights reserved.
  • Published In

    Digital Object Identifier (doi)

    Pubmed Id

  • 16936356
  • Author List

  • Novák J; Čurdová E; Jechová V; Cimburková E; Vaněk Z
  • Start Page

  • 261
  • End Page

  • 266
  • Volume

  • 37
  • Issue

  • 4