Attachment of a fluorescent label to 4-thiouracil and 4-thiouridine

Academic Article

Abstract

  • The conversion of 4-thiouracil and 4-thiouridine into fluorescent derivatives has been carried out using 4-bromomethyl-7-methoxy-2-oxo-2H-benzopyran. Adsorption of the water-insoluble reagent onto Celite allowed the reaction to be applied to completely aqueous media. The conditions employed for the reaction, pH 8.3-8.4 and 37°, are such as to allow ready extension to a tRNA. The blue-white fluorescence of the product (λmax = 400 nm) is visible down to concentrations on the order of 10-7 M. © 1971.
  • Digital Object Identifier (doi)

    Pubmed Id

  • 10780450
  • Author List

  • Secrist JA; Barrio JR; Leonard NJ
  • Start Page

  • 1262
  • End Page

  • 1270
  • Volume

  • 45
  • Issue

  • 5