Purification of Human S-Adenosylmethionine Decarboxylase Expressed in Escherichia coli and Use of This Protein To Investigate the Mechanism of Inhibition by the Irreversible Inhibitors, 5ʹ-Deoxy-5ʹ-[(3-hydrazinopropyl)methylamino] adenosine and 5ʹ-{[(Z)-4-Amino-2-butenyl]methylamino}-5ʹ-deoxyadenosine

Academic Article

Abstract

  • Human S-adenosylmethionine decarboxylase (AdoMetDC) was expressed in high yield in Escherichia coli using the pIN-III(lppp-5) expression vector and purified to apparent homogeneity using affinity chromatography on methylglyoxal bis(guanylhydrazone)-Sepharose. The inactivation of the purified enzyme by 5ʹ-deoxy-5ʹ-[(3-hydrazinopropyl)methylamino]adenosine (MHZPA) was accompanied by an increase in absorbance at 260 nm of the large subunit. This increase was equivalent to the addition of 1 molecule of MHZPA. After digestion with the protease Lys-C, a peptide that contained the bound MHZPA was isolated and found to have the amino acid composition consistent with that expected from the amino terminus of the large subunit. These results indicate that MHZPA inactivates AdoMetDC by forming a hydrazone derivative at the pyruvate prosthetic group. Inactivation of AdoMetDC by 5ʹ-{[(Z)-4-amino-2-butenyl]methylamino}-5ʹ-deoxyadenosine (AbeAdo) led to the appearance of a new peptide peak in the Lys-C protease digest. This peptide had the sequence ASMFVSK. This agrees with the expected sequence from the amino terminus, which is pyruvoyl-SMFVSK, with the exception that the pyruvate has been converted to alanine. Direct gas-phase sequencing of the large subunit of the enzyme also indicated the presence of alanine at the amino terminus after inactivation with AbeAdo. These results indicate that this inhibitor leads to transamination of the pyruvate prosthetic group. Since the pyruvate is covalently linked to the protein, its replacement by alanine leads to an irreversible inactivation of AdoMetDC. © 1992, American Chemical Society. All rights reserved.
  • Published In

  • Biochemistry  Journal
  • Digital Object Identifier (doi)

    Pubmed Id

  • 10625301
  • Author List

  • Shantz LM; Stanley BA; Pegg AE; Secrist JA
  • Start Page

  • 6848
  • End Page

  • 6855
  • Volume

  • 31
  • Issue

  • 29