Fluorescent modification of adenosine 3',5'-monophosphate: spectroscopic properties and activity in enzyme systems.

Academic Article

Abstract

  • The synthesis of a highly fluorescent analog of adenosine 3',5'-monophosphate, namely, 1,N(6)-ethenoadenosine 3',5'-monophosphate, has provided a powerful probe for systems involving adenosine 3',5'-monophosphate. The potential utility of this analog is indicated by its long fluorescent lifetime, detectability at low concentration, and relatively long wavelength of excitation (300 nanometers). In protein kinase systems it is a highly acceptable substitute for adenosine 3',5'-monophosphate.
  • Published In

  • Science  Journal
  • Keywords

  • Acetaldehyde, Acetals, Adenosine Triphosphate, Animals, Cattle, Chemical Phenomena, Chemistry, Chlorine, Chromatography, Thin Layer, Cyclic AMP, Enzyme Activation, Fluorescence, Imidazoles, Muscles, Myocardium, Phosphoric Diester Hydrolases, Phosphorus Isotopes, Phosphotransferases, Protein Binding, Proteins, Rabbits, Spectrophotometry, Structure-Activity Relationship, Tritium, Ultraviolet Rays
  • Pubmed Id

  • 10896725
  • Author List

  • Secrist JA; Barrio JR; Leonard NJ; Villar-Palasi C; Gilman AG
  • Start Page

  • 279
  • End Page

  • 280
  • Volume

  • 177
  • Issue

  • 4045