Synthesis of terminal nucleoside phosphates and thiophosphates via phosphoramidite chemistry

Academic Article

Abstract

  • Synthetic oliqonucleotides for use in biological processes often require a 5'terminal phosphate function. To study enzymatic reactions this group can be replaced by a thiophosphate function. Most chemical methods for the synthesis of these termini base on phosphotriester chemistry. © 1987, Taylor & Francis Group, LLC. All rights reserved.
  • Published In

    Digital Object Identifier (doi)

    Pubmed Id

  • 12091112
  • Start Page

  • 537
  • End Page

  • 539
  • Volume

  • 6
  • Issue

  • 1-2