Enhanced transformation of plant cells following co-bombardment of VirE2 protein of Agrobacterium tumefaciens with DNA substrate

Academic Article

Abstract

  • Agrobacterium tumefaciens transfers a segment of its Ti plasmid (T-DNA) to the nucleus of the host plant cell in single-stranded form. Of the two specific proteins, VirE2, that contains a nuclear localization signal plays a major role to mediate transport of target DNA into the nucleus of the plant cell. Here we report a DNA delivery method termed 'Vir-biolistic' that utilizes this property of the VirE2 protein for delivery of DNA target into the plant cell nucleus through microprojectile bombardment. Transient expression assay using gus reporter gene in immature wheat and maize embryos indicated higher number of transformants with Virbiolistic compared to the popular 'biolistic' procedure of DNA delivery used for the production of transgenic plants. We also found that in wheat calluses co-bombarded with VirE2 protein, the stable integration frequencies of gus genes were threefold higher compared to the biolistic method. Thus, the Vir-biolistic method has the potential to increase the transformation efficiency in plants that are not amenable to Agrobacterium-mediated transformation.
  • Authors

    Published In

  • Current Science  Journal
  • Pubmed Id

  • 2918265
  • Author List

  • Gopalakrishna S; Singh P; Singh NK
  • Start Page

  • 1343
  • End Page

  • 1347
  • Volume

  • 85
  • Issue

  • 9