Objective: To investigate the serotypes and antimicrobial resistance of serogroup 6 Streptococcus pneumoniae (S. pneumoniae) isolated from children with upper respiratory infection in Beijing from 1997 to 2007. Methods: The serogroup 6 and type 6A and 6B of the S. pneumoniae isolates were determined by the quellung reaction using antiserum. Serotype 6C was determined by the multi-bead assay. The minimum inhibitory concentration (MIC) was determined by E-test method for penicillin, amoxicillin-clavulanic acid, ceftriaxone, cefuroxime, erythromycin, imipenem, vancomycin and levofloxacin. Results: A total of 893 S. pneumoniae strains were isolated from 1997 to 2007, and 119 (13.3%) strains were identified as serogroup 6. The number (proportion) of type 6A, 6B and 6C were 71 (59.7%), 39 (32.7%) and 9 (7.6%), respectively. The non-susceptibility rate to penicillin, cefuroxime and erythromycin increased from 3.4%, 1.7% and 86.2% during 1997-2000 to 29.4%, 23.5% and 100% during 2005-2007, respectively. The values of MIC90and the maximum value of MIC of the three antibiotics also increased. The non-susceptibility rate of type 6A, 6B and 6C to penicillin was 7. 2%, 24.3% and 0, respectively. Conclusions: Type 6A was the main type of serogroup 6 S. pneumoniae isolated from children in Beijing. The recently reported type 6C can be found, but less frequently than other types. The resistance of serogroup 6 S. pneumoniae to β-lactams and macrolide antibiotics is more common than before. Type 6B is more resistant than type 6A and 6C.