The determination of a calcium-dependent binding constant of the bovine prothrombin GLA domain (residues 1-45) to phospholipid vesicles

Academic Article

Abstract

  • Calcium-mediated binding of the radioiodinated peptide representing residues 1-45 of bovine prothrombin to single bilayer phospholipid vesicles composed of phosphatidylserine from bovine brain and synthetic 1-palmitoyl-2-oleoyl-phosphatidylcholine (25:75 PS/PC) has been studied over peptide concentrations from 0.33 μM to 3.75 μM and at a calcium concentration of 1.0 mM. The binding isotherm for the interaction between the radioiodinated peptide and PS/PC vesicles fits a model in which there is noncooperative binding of the peptide to non-interacting sites on the phospholipid bilayer. A dissociation constant determined at these conditions is 11.8 μM compared to 1.0 μM for prothrombin fragment 1. © 1988 Academic Press, Inc.
  • Digital Object Identifier (doi)

    Author List

  • Weber DJ; Pollock JS; Pedersen LG; Hiskey RG
  • Start Page

  • 230
  • End Page

  • 235
  • Volume

  • 155
  • Issue

  • 1