The genomic landscape of TERT promoter wildtype-IDH wildtype glioblastoma.

Academic Article

Abstract

  • The majority of glioblastomas can be classified into molecular subgroups based on mutations in the TERT promoter (TERTp) and isocitrate dehydrogenase 1 or 2 (IDH). These molecular subgroups utilize distinct genetic mechanisms of telomere maintenance, either TERTp mutation leading to telomerase activation or ATRX-mutation leading to an alternative lengthening of telomeres phenotype (ALT). However, about 20% of glioblastomas lack alterations in TERTp and IDH. These tumors, designated TERTpWT-IDHWT glioblastomas, do not have well-established genetic biomarkers or defined mechanisms of telomere maintenance. Here we report the genetic landscape of TERTpWT-IDHWT glioblastoma and identify SMARCAL1 inactivating mutations as a novel genetic mechanism of ALT. Furthermore, we identify a novel mechanism of telomerase activation in glioblastomas that occurs via chromosomal rearrangements upstream of TERT. Collectively, our findings define novel molecular subgroups of glioblastoma, including a telomerase-positive subgroup driven by TERT-structural rearrangements (IDHWT-TERTSV), and an ALT-positive subgroup (IDHWT-ALT) with mutations in ATRX or SMARCAL1.
  • Authors

    Published In

    Keywords

  • Adolescent, Adult, Aged, Aged, 80 and over, Brain Neoplasms, Cell Line, Tumor, DNA Helicases, Female, Genomics, Glioblastoma, HEK293 Cells, HeLa Cells, Humans, Isocitrate Dehydrogenase, Male, Middle Aged, Mutation, Promoter Regions, Genetic, Survival Analysis, Telomerase, Telomere Homeostasis, Young Adult
  • Digital Object Identifier (doi)

    Author List

  • Diplas BH; He X; Brosnan-Cashman JA; Liu H; Chen LH; Wang Z; Moure CJ; Killela PJ; Loriaux DB; Lipp ES
  • Start Page

  • 2087
  • Volume

  • 9
  • Issue

  • 1