A simplified procedure for the isolation of bile acids from serum based on a batch extraction with the non-ionic resin--Amberlite XAD-7.

Academic Article

Abstract

  • A simple and reproducible method using the non-ionic resin, Amberlite XAD-7, for the isolation of bile acids from serum by a batch procedure is described. Recoveries were greater than 95% for the non-sulphated bile acids and greater than 70% for the sulphate esters of bile acids. By using 1 g of resin, recoveries were independent of the mass (0.1-5 mumol) of the bile acid present. Up to 35 samples a day can be extracted without requiring dedication of the operator. When serum extracts were analysed by the 3alpha-hydroxysteroid dehydrogenase procedure for estimation of bile acids, virtually all the background fluorescence was eliminated. These extracts were also suitable for gas liquid chromatography, thin layer chromatography, and radioimmunoassay.
  • Published In

    Keywords

  • Bile Acids and Salts, Chenodeoxycholic Acid, Cholic Acids, Deoxycholic Acid, Glycocholic Acid, Humans, Hydroxysteroid Dehydrogenases, Lithocholic Acid, Methods, Resins, Plant, Taurocholic Acid
  • Digital Object Identifier (doi)

    Pubmed Id

  • 23429876
  • Author List

  • Barnes S; Chitranukroh A
  • Start Page

  • 235
  • End Page

  • 239
  • Volume

  • 14
  • Issue

  • 4