Molecular cloning of the t complex responder genetic locus

Academic Article


  • Although mouse t haplotypes carry recessive mutations causing male sterility and embryonic lethality, they persist in wild mouse populations via male transmission ratio distortion (TRD). Genetic evidence suggests that at least five t-haplotype-encoded loci combine to cause TRD. One of these loci, called the t complex responder (Tcr), is absolutely required for any deviation from Mendelian segregation to occur. A candidate for the Tcr gene has previously been identified. Evidence that this gene represents Tcr is its localization to the appropriate genomic subregion and testis-specific expression pattern. Here, we report the molecular cloning of the region between recombinant chromosome breakpoints defining the Tcr locus. These results circumacribe Tcr to a 150- to 220-kb region of DNA, including the 22-kb candidate responder gene. This gene and two other homologs were created by large genomic duplications, each involving segments of DNA 10-fold larger than the individual genes. © 1990.
  • Authors

    Published In

  • Genomics  Journal
  • Digital Object Identifier (doi)

    Pubmed Id

  • 6692440
  • Author List

  • Rosen LL; Bullard DC; Silver LM; Schimenti JC
  • Start Page

  • 134
  • End Page

  • 140
  • Volume

  • 8
  • Issue

  • 1