Gene targeting for inflammatory cell adhesion molecules

Academic Article

Abstract

  • Using gene targeting in mouse embryonic stem cells, it is possible to introduce diverse mutations into specific genes. Using these methods, various laboratories have reported mutations for a variety of inflammatory cell adhesion molecules including CD18, α5 integrin, ICAM-1, P-selectin, and L-selectin; preliminary reports of other mutations are also available. Mutations in CD18 and ICAM-1 cause impaired inflammatory and immune responses, mutations in P-selectin and L-selectin cause decreased leukocyte rolling and emigration, and a mutation in α5 integrin causes embryonic lethality. Gene targeting complements other approaches for analyzing the function of inflammatory cell adhesion molecules.
  • Authors

    Published In

    Author List

  • Bullard DC; Sandberg ET; Scharffetter-Kochanek K; Beaudet AL
  • Start Page

  • 143
  • End Page

  • 154
  • Volume

  • 47