Chloroquine inhibits macrophage tumour necrosis factor-α mRNA transcription

Academic Article

Abstract

  • Although chloroquine administration in vivo following haemorrhage in mice decreases tumour necrosis factor-α (TNF-α) release by macrophage (M∅), the mechanism remains unknown. To study this, peritoneal M∅ (pM∅) from unmanipulated, sham-operated and post-haemorrhage mice were isolated, treated with 0.13 mg/ml chloroquine for 2 hr, and then stimulated with lipopolysaccharide (LPS) for 48 hr. Pretreatment of pM∅ from various groups of mice with chloroquine resulted in 75-90% inhibition of TNF-α release, determined by bioassay. Total RNA was isolated from pM∅ and murine M∅-derived cell lines (P388D1 and RAW 264.7), stimulated with LPS for 0.5 or 1 hr, respectively, and Northern blot analysis for TNF-α mRNA performed. Chloroquine inhibited TNF-α mRNA expression without interfering with mRNA stability, suggesting that this agent reduces M∅ TNF-α release by disrupting TNF-α gene transcription.
  • Authors

    Published In

  • Immunology  Journal
  • Author List

  • Zhu X; Ertel W; Ayala A; Morrison MH; Perrin MM; Chaudry IH
  • Start Page

  • 122
  • End Page

  • 126
  • Volume

  • 80
  • Issue

  • 1